The Southeast Regional Collaborative Access Team (SER-CAT) is an organization consisting of 21 member institutions, formed in 1997 to provide third generation x-ray capabilities to macromolecular crystallographers and structural biologists in the southeastern region of the U.S. 

Emphasis is placed on de novo SAD/MAD structure determination, high-resolution structural analyses, large unit cells, drug design, structural genomics, soft X-ray data collection and next-generation beamline automation. SER-CAT is located at the Advanced Photon Source, Argonne National Laboratory, and is operated by the University of Georgia, with Professor Bi-Cheng Wang as Director.

Please peruse the pages of our website to find out more about SER-CAT and how we are "shining a new light" on protein crystallography.  Have a look at The SER-CAT Spectrum, our newsletter, to learn about recent breakthroughs.  As always, your comments and suggestions are welcome. 

Thank you for visiting! 

 

*News and Announcements*

General Information

SER-CAT ASSOCIATE MEMBERSHIPS

 

Associate Memberships

 

Associate memberships are now available on a yearly basis with no capital; investment or long-term contractual commitment. Associate memberships are available to both academic and industrial users and provide full access to SER-CAT's outstanding Remote Access program at an attractive cost.

 

For more information, please contact SER-CAT Administrative Coordinator, Ms. Kathy S. Savage at kmorris@BCL4.bmb.uga.edu

 

 

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SER-CAT MEETINGS

 

 The 12th SER-CAT Symposium and Board Meeting

 

The 12th SER-CAT Symposium will be help at the University of Pittsburgh on March 13, 2015 and the SER-CAT Executive Board Meeting will be held on March 14, 2015.

 

The local host will be Dr. Bill Furey at the University of Pittsburgh.

 

For more details, please check abck this website.

 

 

 

Past Symposia

 

The joint Mid-Atlantic Macromolecular Crystallography Meeting and the 11th SER-CAT Symposium (http:www.mid-atlantic.org) were held from April 23 - 26, 2014 at the University of Maryland's IBBR Center located on the Shady Grove Campus.

 

The meeting included the following eight sessions: Immunity, Enzymes, SER-CAT, DNA/RNA Enzymes, Methods, Regulatory Proteins, Posters, and a Phenix workshop.

 

During the SER-CAT session, the 2014 SER-CAT Outstanding Science Award Winner, Dr. Samuel Wilson (NIEHS/NIH), presented a talk on "Time-lapse crystallograpy of DNA polymerase beta", and the 2014 SER-CAT Young Investigator Award Winner, Dr. Edward Pryor (University of Virginia), gave a talk entitled, "Doors Windows, and a Roomy Interior: The integral membrane CaaX Protease Ste24p"

 

Additional talk descriptions and meeting information is available on the "Program" and in Dr. John Rose's contributed article in the ACA's Summer 2014 publication of "ACA Structure Matters" (Page 28 - 30, 2014).

 

The 2014 SER-CAT Executive Board Meeting was held on April 26, 2014 at the Quality Suites Inn in Rockville, Maryland.

 

SER-CAT would like to express its sincere thanks t the meeting's organizers, Drs. Roy Mariuzza (University of Maryland, IBBR), Peter Sun (NIAID, NIH), John P. Rose 9SER-CAT, University of Georgia), Osnat herzberg (University of Marylad, IBBR), jeff Boyington (VCR, NIAID, NIH), and Zheng-Qing Fu (SER-CAT, University of Georgia) for planning and providing such an outstanding research program. Special thanks also to the University of Maryland, and its IBBR facility and staff for assisting with the meeting's activities and our attendees and speakers for joining us in this year's event.

 

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SER-CAT AWARDS

CONGRATULATIONS to SER-CAT's 2014 Award Winners!!

Dr. Samuel Wilson, NIEHS-NIH was honored with the 2014 SER-CAT Outstanding Award based on his publication "Observing a DNA polymerase choose right from wrong"

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Dr. Samuel Wilson receiving the 2014 SER-CAT Outstanding Science Award from Dr. John Rose

 

Dr. Edward Pryor, University of Virginia, received the 2014 SER-CAT Young Investigator Award for his publicatio " Structure of the integral membrane protein CAAX protease Ste24p".

Dr. Edward Pryor receiving the 2014 SER-CAT Young Investigator Award from Dr. John Rose. 

 

SER-CAT is pleased to recognize Dr. Wilson and Dr. Pryor for their scientific achievements, and congratulates them again for receiving the 2014 science awards. A special thanks also to all of you who submitted nominations for this year's  presentations.

Click here for a list of previous SER-CAT Award Winners

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2014 SER-CAT Awards nomination forms are available at the following links:

2015 SER-CAT Outstanding Science Award

2015 SER-CAT Young Investigator Award

 

GENERAL USER INFORMATION

New Rayonix MX300HS

The detector is fast (10Hz) however 22BM is flux limited so collecting data at 10 Hz is not recommended.

The best data is collected in shutterless mode which has been incorporated into SERGUI.

For example, a 720 image data set (1 degree images, one second exposure using 1 X-rays) on crystals of bovine trypsin was collected in 12 minutes.

The data were autoprocessed with cmdXDS.

The scaled structure factors were then used with the PHENIX AutoSol workflow to solve the S-SAD (14 sulfurs, 224 residues) 1 X-rays. PHENIX solved the structure, HySS found the 14 sulfurs in the protein, 2 sulfate molecules and a bound calcium.  (delta F" for sulfur is 0.23 and calcium is 0.626 at this wavelength).

PHENIX solved the structure, HySS found the 14 sulfurs in the protein, 2 sulfate molecules and a bound calcium.  (delta F" for sulfur is 0.23 and calcium is 0.626 at this wavelength).

A total of 202 of the 224 residues were fit and the current model after AutoSol has an F-factor 0.2054 (Rfree = 0.2314).

A data collection guide has been prepared and may be downloaded by clicking the following link:

Rayonix MX300HS Users Guide

Please look it over since there are several changes in what you will need to do to collect data and process data with the new detector.

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HKL2000 now availabel for the MX300HS

A new version of HKL2000 that can process the data from the MX300HS detector is available for download from HKL Research.  Please contact them for download instructions.  You will alos need to get an updated cr_info file.

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Data Archiving

Users should avoid bringing firewire drives with metal cases for use at the beamlines.

The metal, being a wonderful heat conductor, is causing thermal failure because of the long download time required for data collection.

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SER-CAT Automounters

Both BM and ID Robots are fully commissioned. All first time robotics users must contact Elizabeth Kolbusz (SER-CAT's User Support Coordinator) prior to their visit.

The ALS Style magnetic caps are available from Hampton Research, P/N HR4-779

The 18mm Mounted litholoops are available from Molecular Dimensions.

The dry shipping dewars compatible with SER-CAT puck holder are from Taylor-Wharton (Model: CX100 or CXR100). The product vendor is: www.discountcryo.com.

SER-CAT's robotic pucks, puck holder and tools are available from the University of Georgia Instrument Shop, 955 East Whitehall Rd., Athens, GA 30602. Please Contact Mr. Lewis Fortner via e-mail (lewisf@uga.edu) or  Fax (706-542-7921) for pricing and availability.

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MISCELLANEOUS MEMBERSHIP INFORMATION

As a service to our Members, SER-CAT is pleased to provide helpful links for the following information:

Lease and/or Sale Opportunities Available by Members

 

Positions Available at SER-CAT and/or Member Institutions

 

Other Membership Announcements

 

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DIRECTOR'S HIGHLIGHTS

Historical Presentation of SER-CAT  SER-CAT celebrated its 10th year Anniversary in 2007. In honor of this special occasion, SER-CAT's director Dr. Bi-Cheng Wang created a historical presentation highlighting many SER-CAT's exciting achievements. You are invited to view this PDF slideshow entitled, "SER-CAT: The First 10 Years" to see the chronological history of our development, including the important ribbon cutting ceremony held at the APS in 2002. We sincerely express our gratitude to each of you who supported SER-CAT's growth, and look forward to many more productive years ahead!

The Beamline dedication ceremony in Argonne, IL

Celebrating the official opening of SER-CAT's 1st beamline, October 2002, APS, ANL

   Photo of the Advanced Photon Source in Argonne, IL.

 

The SER-CAT Approach!

 

We would like to share another slide presentation given by SER-CAT Director Dr. Bi-Cheng Wang at the 9th International Conference on Biology and Synchrotron Radiation in Manchester, England, entitled, "Remaining Challenges in Long Wavelength Phasing: The SER-CAT Approach" (PDF). This presentation highlights SER-CAT's work on data collection at all possible wavelengths, including sulfur phasing.

 

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OTHER USEFUL INFORMATION

 

SER-CAT Featured on World Business Review

 

SER-CAT was featured on the TV program World Business Review, which aired on the Bravo Network in February 2005. You may view the featured segment in streaming audio following the links below. If you would like a copy of this valuable resources to distribute locally ob CD or DVD, please contact Lily Li at lily@secsg.uga.edu.

 

Windows Media video (300K)
Real Player video (300K)